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KMID : 1149220060140020086
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Korean Journal of Oriental Medical Prescription
2006 Volume.14 No. 2 p.86 ~ p.96
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A Study on the Short Term Effect of Rossa rugosae Radix on Proliferation, Differentiation & Maturation of 3T3-L1 Preadipocyte
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Park Jong-Hyo
Kim Dong-Woo
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Abstract
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Objectives : This study wad designed to elucidate the short term effect of Rossa rugosae Radix on proliferation, differentiation and maturation of 3T3-L1 Preadipocyte.
Methods : 3T3-L1 preadipocytes obtained from Korean Cell line Bank were cultured in a Dulbecco’s modified eagle medium(DMED) culture colution containing 10% fetal bovine serum(FBS) and various concentration of aqueous extract of Rossa rugosae Radix on proliferation, differentiation and maturation of 3T3-L1 preadipocytes were investigate after treatment for 24 hours b measuring MTT, Oil Red O and latate dehydrogenase activity..
Results : The Rossa rugosae Radix extract inhibited significantly the proliferation of 3T3-L1 preadipocytes and tended to increase latate dehydrogenase activity in the media of differentiated 3T3-L1 preadipocytes & matured 3T3-L1 preadipocytes. the extract also inhibit the lipid accumulation of differentiated and maturaion of 3T3-L1 preadipocytes, suggesting that Rossa rugosae Radix has anti-obesity effect: however further in vivo study is needed to demonstrate its pharmacological effects.
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KEYWORD
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Rossa rugosae Radix, 3T3-L1 Preadipocyte, LDH, anti-obesity
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